Ultraviolet light irradiation of PM2 superhelical DNA
نویسندگان
چکیده
منابع مشابه
Discrete Fragments Produced by Limited Digestion of Superhelical PM2 DNA with Venom Phosphodiesterase
Limited digestion of superhelical (form I) PM2 DNA by venom phosphodiesterase yields unit length linear (form III) molecules plus a series of discrete fragments. The positions of eight of these fragments on the genome map were determined with the use of restriction endonucleases Hpa II and HindIII. These positions were compared with the locations of the eight early denaturation regions in form ...
متن کاملCarbodiimide modification of superhelical PM2 DNA: considerations regarding reaction at unpaired bases and the unwinding of superhelical DNA with chemical probes.
Superhelical PM2 DNA I can be modified with N-cyclohexyl-N'-beta-(4-methylmorpholinium)ethyl carbodiimide (CMC). The transition of the sedimentation coefficient uncorrected for buoyant density change (S20,*) vs. % reactivity in terms of base pairs shows the following characteristics. The S20,* increases by 4.5 S units upon 1% modification. There is a plateau in S20,* between 1 and 4% reactivity...
متن کاملUltraviolet Light Irradiation of Pm2 Superhelical Dna "when I Was Your Age, I Always Did It for Half-an-hour a Day. Why, Sometimes I've Believed as Many as Six Impossible Things before Breakfast..." from through the Looking-giass
Superhelical PM2 DNA can be photochemically modified by u.v. irradiation. The variation of S 2 0 with dose shows the following characteristics. There is a linear increase from 28 to 31S produced by a low dose of u.v. irradiation (4,000 ergs/mm). A plateau in S~Q occurs between 4,000 and 10,000 ergs/mm. The S 2 n then increases when'irradiation is increased to 56,000 ergs/mm. Thymine dimers are ...
متن کاملAntibacterial response of Cd-TiO2/PEG/folic acid nanocomposite under ultraviolet, visible light, or ultrasonic irradiation
Synthesis and characterization of Cd-TiO2/PEG/FA nanocomposite as a biomaterial is the main aim of this research. Cd-doped TiO2 nanoparticles (NPs) were synthesized by solvothermal assisted sol-gel method. Then, polyethylene glycol (PEG) was added to the as-synthesized NPs in order to modify their surface and to prevent agglomeration. In the next step, folic acid (FA) was conjugated to Cd-TiO2/...
متن کاملChanges induced by ultraviolet light in the superhelical DNA of lymphocytes from subjects with xeroderma pigmentosum and normal controls.
Patients with the light-sensitive disease xeroderma pigmentosum (XP) are genetically heterogeneous. Most patients are defective in the excision repair of u.v.-induced DNA damage while some, the XP-variants, seem proficient in excision repair but replicate u.v.-damaged DNA with difficulty. The former have so far been assigned to 5 complementation groups (A-E) of which B and E are extremely rare....
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1977
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/4.5.1243